Module 1 – Fundamental
HeF000 – Theoretical presentation of In Vitro sun protection assessment (Theory)

Goal:
- Testing methods In Vitro and In Vivo comparison
- Technique and limits
- Results interpretation
Session:
- 0.5 day
- 5 trainees
- In your office or in our laboratory
Presentation of In Vitro test
- Principle of solar protection
- How to protect (Absorption – Scaterring)
- Mecanisms involved and evaluation methods
Understand In Vitro test
- In Vitro & In Vivo
- Key parameters of In Vitro test (Appliances – Substrate – Calibration)
- Traps to avoid for valid results (Methods – Products – Artefacts)
- Why In Vitro test?
Methods
- In Vivo / In Vitro correlation: Why? How? Limits?
- Added value of methods (global protection – comparion)
Practice part
- No treated in this module
Module 2 – Deepening
HeF001b – UV transmission and evaluating sun protection (Theory + Practice)

Goal:
- Existing methods, their specificities
- Testing: conditions, control, data treatment
Session:
- 1 – 2 days
- 3 – 5 trainees
- In your office or in our laboratory
Unterstand In Vitro test
- Impact of Technical & Reglementation aspect on In vitro method development
- Prerequisite of method validation (Difference – Advantage – Inconvenience)
Relevance of results
- Methodology of each method
- Key parameters of In vitro test
- Traps to avoid for valid results (Methods – Products – Artefacts)
Methods
- How to approach test and ensure reproducibility?
- Self-validation & control
- In Vivo / In Vitro correlation & pre-irradiation (study example)
- Standard method ISO 24443, FDA 2011 and Boots Star Rating system 2011
- Photostability & pre-irradiation
Practice part
- Operational conditions: Tool – Appliance – Sample – Calibration
- Steps of In Vitro test: Application – Spreading – Measurement
- Data acquisition: Treatment – Interpretation
Module 3 – Development
HeF002b – Mastering sun protection evaluation / specific method (Theory + Practice)

Goal:
- Your specific questions
- Your laboratory practical difficulties
- The specific methods
Session:
- 1 – 2 days
- 3 – 5 trainees
- In your office or in our laboratory
Understand In Vitro test
- Reasons of reproducibility difficulties
- Know how to deflect practical difficulties: Advantage – Inconvenience
Relevance of results
- Diversity of methods
- How ensure best use of in vitro results
- Evaluation method through measurement difficulties
Methods
- Standard ISO 24443
- Standard FDA 2011
- Method Boots Star Rating System 2011
- Photostability and pre-irradiation
Practice part
- Operational conditions: Tool – Appliance – Sample – Calibration
- Steps of In Vitro test: Application – Spreading – Measurement
- Data acquisition: Treatment – Interpretation
Your issues
- Questions
- Specific methods
- Practical lab difficulties